Mass Spectrometry and Chromatography

Proteomic Experiment: Mass Spectrometry Cellular factories localize and push proteins into compartments where they perform their variety of the functions and subcellular protein provide high-end indication for its activities. Researchers can envisage protein dispersal in the cell by using Super-resolution microscopy by applying antibodies or by tagging proteins with florescent probes. Such kind of method is not innovative and researchers have to pin down their choice of proteins to a known set. Unbiased Mass Spectrometry based proteomic method present expansive way, and the credit goes to the specificity, accuracy and scale they provide. Lately researchers are using the approach to learn protein activity at the sub cellular level. The new dubbed spatial proteomic methodology, offer researchers to build a comprehensive cellular maps and show the hidden life of proteins- who they interact with, where they live & they move around- in healthy as well as in diseased cells. We ...

Divine Sanction for Analytical World: Mass Spectrometry and Chromatography Mass spectrometry is a revolution to the analytical world which have boosted up the analytical market due to its wonderful characteristic of quantitative and qualitative determination. Mass spectrometry has made the analysis very simpler and broader by its superior feature which is irreplaceable. Mass spectrometry plays its role in every stage of analysis and is the major tool in every field from medicine to agriculture. This technique is getting modified in every corner of this technical world. Researchers are further modifying these techniques to be applied for different fields. Without this technique the analytical world is incomplete as this is the only technique which solve the queries of the experiments by giving appropriate result. In tumorigenesis, role of histidine phosphorylation is the most recent experiment. Histidine phosphorylation is the hidden phosphoproteome. When 12 tumours are pro...

Chromatography and Spectrometry: Revolution in Agriculture In today’s ultramodern day the demand and existence of analytical tools are getting broad day by day. Mass spectrometry is at its cornerstone in such technical world as it is required from clinical to an agricultural field. There is no corner in which these techniques are not required. These techniques are non-invasive and superfast methods for the monitoring of the materials and its constituents. In agricultural and food industries these techniques are applied for the monitoring and analysing the quality of the raw material used, end product obtained to the soil quality. Greenness defining, infection detection, mass determination, chemical compound evaluation, moisture estimation and other analysis can be done by spectrometry techniques. For the agricultural purpose, these techniques are proved to be fast, adequate, noncontact, and low cost. Perhaps these techniques are appropriate alternative and intelligent instrumen...

SOLICITATION OF IONIZATION TECHNIQUE IN MASS SPECTROMETRY There are many ionization techniques combined with Mass spectrometry.  Electron impact (EI) and  Fast Atom Bombardment (FAB) are the classical ionization technique used mainly by the chemist. Now, these  techniques for ionization  are not generally used in modern mass spectrometry except EI for environmental work using GC-MS.   Atmospheric Pressure Chemical Ionization (APCI), electrospray ionization (ESI), Matrix Assisted Laser Desorption Ionization (MALDI) are Modern Ionization techniques in mass spectrometry. APCI forms a protonated molecule and is completely compatible with  Liquid Chromatography  (LC) and so it is more preferred over EI methods. Electrospray Ionization Method Combined with  Matrix-assisted laser desorption ionization  (MALDI) has eliminated the application of FAB because it produces the protonated molecules, which made FAB v...

Clinical significance of MS In the clinical laboratories for the diagnosis of disease, disease screening and metabolic disorders, monitoring of the drug therapy, identifying the drug poisoning and toxicity and regulation of the advanced biomarkers the mass spectrometry technology have been used. By the application of the mass spectrometry technique the therapeutic regulation of immunosuppressant drugs is well established. For the evaluation of the steroidal hormones for the endocrine disorders and vitamin D, the liquid chromatography-mass spectrometry is the best technique in the clinical laboratories. The tandem mass spectrometry is used for the measurement of the thyroid hormones which have overcome the issue with immunoassays. For the toxic drug quantitation and toxicology screening the triple quadrupole mass spectrometry is preferred. In clinical microbiology laboratories for the rapid identification of the microorganism automated MALDI-TOF MS technology is applied. For...