Proteomic Experiment: Mass Spectrometry

Cellular factories localize and push proteins into compartments where they perform their variety of the functions and subcellular protein provide high-end indication for its activities. Researchers can envisage protein dispersal in the cell by using Super-resolution microscopy by applying antibodies or by tagging proteins with florescent probes. Such kind of method is not innovative and researchers have to pin down their choice of proteins to a known set.

Unbiased Mass Spectrometry based proteomic method present expansive way, and the credit goes to the specificity, accuracy and scale they provide. Lately researchers are using the approach to learn protein activity at the sub cellular level. The new dubbed spatial proteomic methodology, offer researchers to build a comprehensive cellular maps and show the hidden life of proteins- who they interact with, where they live & they move around- in healthy as well as in diseased cells. We can evaluate protein abundance and half-life of the protein, but spatial proteomics was still missing the most important question in the brain for the functioning of the protein. Neuron hold more real estate per cell as compared to the other types of the cell and distribution of protein is wide from the centre body or soma by extensions known as axons, to the far reaching cellular functions known as dendrites that ends at the synapses and hold in the communication of the internal neuron.

Isolation of highly enriched cell fractions is one of the challenge in the spatial proteomic usually from small amounts of the beginning material, and after that to separate the organelles with minimum contamination by the other cellular structure. When dealing with the brain tissues that challenge multiplies where the diversity of cell is high and so amount of different cell types are negligible. By mass spectroscopy scientist are braving these odds to map thousands of proteins within the nook of the brain and they are achieving their targets and doing discovery in the analytical field.

Mass spectrometry and chromatography are the keys to success for all the experiment challenges  in the analytical field and we should appreciate them as analysis is incomplete without these techniques. All such interesting discussion will be held by the researchers regarding their successful experiments and research work in the upcoming 10^th International Conference on Mass Spectrometry and Chromatography Conference during October 08-09, 2018 in Dubai, UAE. This conference will be the extra-ordinary platform of experts and boost up the knowledge by discussing unknown facts of their research work. Researchers, knowledge seekers and others experts can be the part of this conference as this conference welcomes you to share and discuss your knowledge all around the world.