CHROMATOGRAPHY TECHNIQUE

Chromatography is a collective set of laboratory technique for the separation of mixtures. In chromatography the sample is dissolved in mobile phase (gas, liquid, or supercritical fluid. Phase is chosen according to the component of the sample having different solubility in different phase to generate differences in the mobility as a result the components get separated as they pass by the stationary phase. This technique is analytical as well as preparative too. The main aim of the preparative chromatography is to separate the component from the mixture for its future application and its purification. And analytical chromatography is usually done with the smaller amount of the sample and measuring the proportion of the analytes in the sample. Previously these techniques were used to separate component on the base of their colours as for herbal pigment. With the passing time and increase in its application its demand is also extended. In today’s world chromatography is accepted as a very sensitive case and an effective tool for the separation method. This technique is the most efficacious for the separation and determination of the sample component. It is a multi-residue technique for the determination of pesticides in vegetables and fruits. Column chromatography technique is used for the protein purification method based on the characteristic features of the proteins and this technique is also used to control the purity of the protein. HPLC (High Performance Liquid Chromatography) technique has the superior feature that include its higher sensitivity, its use as quantitative method, rapid turnover rate, and ability to purify amino acids hydrocarbons, nucleic acids, carbohydrates, drugs, steroids and antibiotic. Chromatography is applied in broad field which cannot be replaced by other techniques and is predicted to be the master tool of analytical field in the future. This technique is extending in other field and increasing its scope and application globally.

Comments

Post a Comment

Popular posts from this blog

Image
Gas chromatography and mass spectrometry- welcome news for chocolate lovers Apart from other research, gas chromatography and mass spectrometry, the heartbeat of analytical tools again proved to be bonus for the chocolate lovers. Serotonin called as mood lifter found in chocolate, which is manufactured by cocoa beans. Chocolate change the mood and is loved by every category of population. Jackfruit found in most of the tropical region which are very low cost and are now replacement for cocoa beans, which is the main constituent in chocolate. The demand of chocolate in the world is elevating day by day and beating the production of cocoa beans. The sweet smelling jackfruit are boiled, roasted and steamed prior to eating, which is the cheap source of protein, fibre, and minerals, and these are mostly thrown as waste. The analysis predicted that the demand for these beans will cross 4 million tonnes by 2020.Researchers were finding a better way to put the waste jackfruits ...
Read more
Image
SOLICITATION OF IONIZATION TECHNIQUE IN MASS SPECTROMETRY There are many ionization techniques combined with Mass spectrometry.  Electron impact (EI) and  Fast Atom Bombardment (FAB) are the classical ionization technique used mainly by the chemist. Now, these  techniques for ionization  are not generally used in modern mass spectrometry except EI for environmental work using GC-MS.   Atmospheric Pressure Chemical Ionization (APCI), electrospray ionization (ESI), Matrix Assisted Laser Desorption Ionization (MALDI) are Modern Ionization techniques in mass spectrometry. APCI forms a protonated molecule and is completely compatible with  Liquid Chromatography  (LC) and so it is more preferred over EI methods. Electrospray Ionization Method Combined with  Matrix-assisted laser desorption ionization  (MALDI) has eliminated the application of FAB because it produces the protonated molecules, which made FAB v...
Read more
Image
Proteomic Experiment: Mass Spectrometry Cellular factories localize and push proteins into compartments where they perform their variety of the functions and subcellular protein provide high-end indication for its activities. Researchers can envisage protein dispersal in the cell by using Super-resolution microscopy by applying antibodies or by tagging proteins with florescent probes. Such kind of method is not innovative and researchers have to pin down their choice of proteins to a known set. Unbiased Mass Spectrometry based proteomic method present expansive way, and the credit goes to the specificity, accuracy and scale they provide. Lately researchers are using the approach to learn protein activity at the sub cellular level. The new dubbed spatial proteomic methodology, offer researchers to build a comprehensive cellular maps and show the hidden life of proteins- who they interact with, where they live & they move around- in healthy as well as in diseased cells. We ...
Read more