Proteomic Experiment: Mass Spectrometry Cellular factories localize and push proteins into compartments where they perform their variety of the functions and subcellular protein provide high-end indication for its activities. Researchers can envisage protein dispersal in the cell by using Super-resolution microscopy by applying antibodies or by tagging proteins with florescent probes. Such kind of method is not innovative and researchers have to pin down their choice of proteins to a known set. Unbiased Mass Spectrometry based proteomic method present expansive way, and the credit goes to the specificity, accuracy and scale they provide. Lately researchers are using the approach to learn protein activity at the sub cellular level. The new dubbed spatial proteomic methodology, offer researchers to build a comprehensive cellular maps and show the hidden life of proteins- who they interact with, where they live & they move around- in healthy as well as in diseased cells. We ...
Popular posts from this blog
SOLICITATION OF IONIZATION TECHNIQUE IN MASS SPECTROMETRY There are many ionization techniques combined with Mass spectrometry. Electron impact (EI) and Fast Atom Bombardment (FAB) are the classical ionization technique used mainly by the chemist. Now, these techniques for ionization are not generally used in modern mass spectrometry except EI for environmental work using GC-MS. Atmospheric Pressure Chemical Ionization (APCI), electrospray ionization (ESI), Matrix Assisted Laser Desorption Ionization (MALDI) are Modern Ionization techniques in mass spectrometry. APCI forms a protonated molecule and is completely compatible with Liquid Chromatography (LC) and so it is more preferred over EI methods. Electrospray Ionization Method Combined with Matrix-assisted laser desorption ionization (MALDI) has eliminated the application of FAB because it produces the protonated molecules, which made FAB v...
10th International Conference on Mass Spectrometry and Chromatography
Liquid chromatography–mass spectrometry Liquid chromatography: Liquid chromatography -hyphenated technique combined with HPLC with the detection power of mass spectrometry in which the components are distributed between two phases. Liquid chromatography can be divided into five categories Adsorption chromatography : Adsorption chromatography enables the use of liquid chromatography to separate a racemic mixture into its enantiomeric parts. Partition chromatography : The stationary phase and the mobile phase in partition chromatography are liquid. The stationary phase liquid would be a homogeneous mixture liquid with the mobile phase. Ion-exchange chromatography : ion exchange chromatography is applied to separate and determine ions on columns that have a low ion exchange capacity and it is based on the equilibrium of ion exchange Size-exclusion chromatography : Size exclusion chromatography separates molecules by their size. Th...
Comments
Post a Comment